Clear-cell carcinomas are some of the most common renal cancers observed in humans. Current medical efforts at finding cures to the cancer focus on the responses of it to the inhibition of RAF-1, VEGR and PDGFR, all found in the Caki-1 cell line. Through transfection experiments with appropriate reagents, researchers can examine the possibilities of genetic modification and its ramifications on the physiology of Caki-1, leading to reliable results that can inform preclinical trials and commercial drug development.
Caki-1 is a tumorigenic cell line that was established from the cells of a renal tumor taken from a 49-year-old Caucasian male with clear cell carcinoma. Caki-1 cells have a modal chromosome number of 68, exhibit an epithelial-like morphology, and grow adherently in monolayers in vitro . The Caki-1 cell line is suitable for both in vivo and in vitro transfection to study kidney cancer, and a Caki-1 xenograft model and Caki-1 Transfection Reagent to transfect Caki-1 cells are available through Altogen Biosystems .
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