Skip to main content

Klotho Inhibits EGF-induced Cell Migration in Caki-1 Cells

Klotho is a transmembrane protein that has been reported to have anti-cancer properties. In this study, researchers analyze the effects of Klotho on EGF-mediated cell migration in Caki-1 cells. Caki-1 cells were treated in vitro with Klotho and cell adhesion, migration, and biochemical studies were conducted to observe the effects. Results showed that Klotho successfully inhibited EGF-induced Caki-1 adhesion, and migration through the inactivation of EGFR and p38 MAPK signaling pathways. These findings suggest that cRCC metastasis could be a result of decreased Klotho expression. [LINK] 

Comments

Post a Comment

Popular posts from this blog

Caki-1 Cell Line

Caki-1 is a tumorigenic cell line that was established from the cells of a renal tumor taken from a 49-year-old Caucasian male with clear cell carcinoma. Caki-1 cells have a modal chromosome number of 68, exhibit an epithelial-like morphology, and grow adherently in monolayers in vitro . The Caki-1 cell line is suitable for both in vivo and in vitro transfection to study kidney cancer, and a Caki-1 xenograft model and Caki-1 Transfection Reagent to transfect Caki-1 cells are available through Altogen Biosystems .
Post a Comment
Read more

Metformin Induces Various Responses in Caki-1 and Caki-2 Cells

Clear cell renal cell carcinoma (ccRCC) is one of the most common urological cancers diagnosed globally. Two cell lines that derive from human ccRCC are Caki-1 and Caki-2, which express wild-type VHL (von Hippel-Lindau). Defining characteristics of ccRCC include the mutation of the VHL tumor suppressor gene and the overexpression of hypoxia-inducible factor (HIF)-1 α protein.  Previous studies have suggested that metformin (a drug used to treat diabetes) exhibit antineoplastic effects in different types of cancers since its activation of AMPK evidently decreases the expression of HIF-1 α.  In this study, researchers analyze the effect of metformin on both Caki-1 and Caki-2 cell lines. Several concentrations of Metformin were used to treat the cell lines, and various assays and analyses were used to observe cell viability, cell cycle arrest, migration, and apoptosis. Results from this study indicate that Metformin inhibited cell growth in both Caki-1 and Caki-2 cell lines. Howe...
Post a Comment
Read more

Kidney Cancer Research Through Caki-1 Cells

Clear-cell carcinomas are some of the most common renal cancers observed in humans. Current medical efforts at finding cures to the cancer focus on the responses of it to the inhibition of  RAF-1, VEGR and PDGFR, all found in the Caki-1 cell line. Through transfection experiments with appropriate reagents , researchers can examine the possibilities of genetic modification and its ramifications on the physiology of Caki-1, leading to reliable results that can inform preclinical trials and commercial drug development.
Post a Comment
Read more