Klotho is a transmembrane protein that has been reported to have anti-cancer properties. In this study, researchers analyze the effects of Klotho on EGF-mediated cell migration in Caki-1 cells. Caki-1 cells were treated in vitro with Klotho and cell adhesion, migration, and biochemical studies were conducted to observe the effects. Results showed that Klotho successfully inhibited EGF-induced Caki-1 adhesion, and migration through the inactivation of EGFR and p38 MAPK signaling pathways. These findings suggest that cRCC metastasis could be a result of decreased Klotho expression. [LINK]
Clear cell renal cell carcinoma (ccRCC) is one of the most common urological cancers diagnosed globally. Two cell lines that derive from human ccRCC are Caki-1 and Caki-2, which express wild-type VHL (von Hippel-Lindau). Defining characteristics of ccRCC include the mutation of the VHL tumor suppressor gene and the overexpression of hypoxia-inducible factor (HIF)-1 α protein. Previous studies have suggested that metformin (a drug used to treat diabetes) exhibit antineoplastic effects in different types of cancers since its activation of AMPK evidently decreases the expression of HIF-1 α. In this study, researchers analyze the effect of metformin on both Caki-1 and Caki-2 cell lines. Several concentrations of Metformin were used to treat the cell lines, and various assays and analyses were used to observe cell viability, cell cycle arrest, migration, and apoptosis. Results from this study indicate that Metformin inhibited cell growth in both Caki-1 and Caki-2 cell lines. Howe...
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