Caki-1 Transfection Reagent (Kidney Epithelial Cells)

Clusterin has been previously reported to promote cell proliferation, migration, and invasiveness in renal cell carcinoma, but the underlying mechanisms have not been established. In this study, researchers analyzed of effects of clusterin on Caki-1 cell growth, invasion and S100A4 expression. Caki-1 cells were treated with shRNA targeting clusterin in vitro , and different assays were used to detect clusterin and S100A4 expression, as well as Caki-1 cell viability and growth. Results found that an overexpression of clusterin increased cell proliferation and invasiveness, which was mediated by the up-regulation of S100A4. Conversely, targeting clusterin led to a decrease in cell proliferation and invasiveness. These findings suggest that suppressing clusterin could be used in future therapeutic approaches for treating renal cell carcinoma. [ LINK ]

Klotho is a transmembrane protein that has been reported to have anti-cancer properties. In this study, researchers analyze the effects of Klotho on EGF-mediated cell migration in Caki-1 cells. Caki-1 cells were treated in vitro with Klotho and cell adhesion, migration, and biochemical studies were conducted to observe the effects. Results showed that Klotho successfully inhibited EGF-induced Caki-1 adhesion, and migration through the inactivation of EGFR and p38 MAPK signaling pathways. These findings suggest that cRCC metastasis could be a result of decreased Klotho expression. [ LINK ] 

Clear cell renal cell carcinoma (ccRCC) is one of the most common urological cancers diagnosed globally. Two cell lines that derive from human ccRCC are Caki-1 and Caki-2, which express wild-type VHL (von Hippel-Lindau). Defining characteristics of ccRCC include the mutation of the VHL tumor suppressor gene and the overexpression of hypoxia-inducible factor (HIF)-1 α protein.  Previous studies have suggested that metformin (a drug used to treat diabetes) exhibit antineoplastic effects in different types of cancers since its activation of AMPK evidently decreases the expression of HIF-1 α.  In this study, researchers analyze the effect of metformin on both Caki-1 and Caki-2 cell lines. Several concentrations of Metformin were used to treat the cell lines, and various assays and analyses were used to observe cell viability, cell cycle arrest, migration, and apoptosis. Results from this study indicate that Metformin inhibited cell growth in both Caki-1 and Caki-2 cell lines. However, Caki

Altogen Biosystems is a recognized provider and laboratory for the pharmaceutical industry. Custom research services and additional goods include xenografts, cell transfection optimization, 28-day generation of stably-transfected cells, IND applications and more. Dedicated to assisting customers with cutting-edge and efficient services, Altogen Biosystems endeavors to advance modern laboratory techniques. Visit the official website for a Caki-1 xenograft model .

Clear-cell carcinomas are some of the most common renal cancers observed in humans. Current medical efforts at finding cures to the cancer focus on the responses of it to the inhibition of  RAF-1, VEGR and PDGFR, all found in the Caki-1 cell line. Through transfection experiments with appropriate reagents , researchers can examine the possibilities of genetic modification and its ramifications on the physiology of Caki-1, leading to reliable results that can inform preclinical trials and commercial drug development.

The Caki-1 cell line was established from the renal carcinoma cells of a 49-year-old male patient with clear cell carcinoma. Caki-1 cells contain many microvilli, small mitochondria,and well developed endoplasmic reticulum (ER) and golgi apparatus. This cell line is not commercially available and is only available for research purposes. Transfection of the Caki-1 cell is facilitated by Caki-1 transfection reagents ( Altogen biosystems )  to establish stable cell lines, produce consistent research and to optimize transfection of siRNA, miRNA and pDNA.  Caki-1 Transfection Reagent (Kidney Epithelial Cells)