Caki-1 is a tumorigenic cell line that was established from the cells of a renal tumor taken from a 49-year-old Caucasian male with clear cell carcinoma. Caki-1 cells have a modal chromosome number of 68, exhibit an epithelial-like morphology, and grow adherently in monolayers in vitro. The Caki-1 cell line is suitable for both in vivo and in vitro transfection to study kidney cancer, and a Caki-1 xenograft model and Caki-1 Transfection Reagent to transfect Caki-1 cells are available through Altogen Biosystems.
Clear cell renal cell carcinoma (ccRCC) is one of the most common urological cancers diagnosed globally. Two cell lines that derive from human ccRCC are Caki-1 and Caki-2, which express wild-type VHL (von Hippel-Lindau). Defining characteristics of ccRCC include the mutation of the VHL tumor suppressor gene and the overexpression of hypoxia-inducible factor (HIF)-1 α protein. Previous studies have suggested that metformin (a drug used to treat diabetes) exhibit antineoplastic effects in different types of cancers since its activation of AMPK evidently decreases the expression of HIF-1 α. In this study, researchers analyze the effect of metformin on both Caki-1 and Caki-2 cell lines. Several concentrations of Metformin were used to treat the cell lines, and various assays and analyses were used to observe cell viability, cell cycle arrest, migration, and apoptosis. Results from this study indicate that Metformin inhibited cell growth in both Caki-1 and Caki-2 cell lines. Howe...
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