Skip to main content

Klotho Inhibits EGF-induced Cell Migration in Caki-1 Cells

Klotho is a transmembrane protein that has been reported to have anti-cancer properties. In this study, researchers analyze the effects of Klotho on EGF-mediated cell migration in Caki-1 cells. Caki-1 cells were treated in vitro with Klotho and cell adhesion, migration, and biochemical studies were conducted to observe the effects. Results showed that Klotho successfully inhibited EGF-induced Caki-1 adhesion, and migration through the inactivation of EGFR and p38 MAPK signaling pathways. These findings suggest that cRCC metastasis could be a result of decreased Klotho expression. [LINK] 

Comments

Post a Comment

Popular posts from this blog

Kidney Cancer Research Through Caki-1 Cells

Clear-cell carcinomas are some of the most common renal cancers observed in humans. Current medical efforts at finding cures to the cancer focus on the responses of it to the inhibition of  RAF-1, VEGR and PDGFR, all found in the Caki-1 cell line. Through transfection experiments with appropriate reagents , researchers can examine the possibilities of genetic modification and its ramifications on the physiology of Caki-1, leading to reliable results that can inform preclinical trials and commercial drug development.
Post a Comment
Read more

Caki-1 Transfection Reagent (Kidney Epithelial Cells)

The Caki-1 cell line was established from the renal carcinoma cells of a 49-year-old male patient with clear cell carcinoma. Caki-1 cells contain many microvilli, small mitochondria,and well developed endoplasmic reticulum (ER) and golgi apparatus. This cell line is not commercially available and is only available for research purposes. Transfection of the Caki-1 cell is facilitated by Caki-1 transfection reagents ( Altogen biosystems )  to establish stable cell lines, produce consistent research and to optimize transfection of siRNA, miRNA and pDNA.  Caki-1 Transfection Reagent (Kidney Epithelial Cells)
Post a Comment
Read more

Caki-1 Cell Line

Caki-1 is a tumorigenic cell line that was established from the cells of a renal tumor taken from a 49-year-old Caucasian male with clear cell carcinoma. Caki-1 cells have a modal chromosome number of 68, exhibit an epithelial-like morphology, and grow adherently in monolayers in vitro . The Caki-1 cell line is suitable for both in vivo and in vitro transfection to study kidney cancer, and a Caki-1 xenograft model and Caki-1 Transfection Reagent to transfect Caki-1 cells are available through Altogen Biosystems .
Post a Comment
Read more